Many of the laboratory procedures involve the use of dilutions. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated. As example, if 1.0 ml of 3M HCl is added with 9 ml of distilled water, the dilution factor becomes 1/10. Serial Dilution: Importance and Aplication. Then, 1 mL from that mixture is added to 9 mL, and it is mixed together (a 10-2 dilution). As shown in the illustration below, it begins when 1 mL of the bacterial sample is added to 9 mL, and it is mixed together (creating a 10-1 dilution). The factor can be obtained by dividing the actual volume of the initial chemical used to the final volume of the chemical after water is added. A serial dilution is the dilution of a sample, in 10-fold dilutions. Ideally, therefore, we would like to get 100 bacteria on the plate that we count. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M. We would like to count the bacteria in a particular sample, and we think that there should be in the neighborhood of 10 million cells per mL. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. One way is by obtaining a factor called the Dilution Factor (DF). A serial dilution is the stepwise dilution of a substance in solution. After performing the dilution, we need to know how much difference are the diluted chemical and the initial, undiluted ones. Cultures are usually allowed to grow through a normal growth curve, with daily transfer of a small volume of the expanded culture into fresh medium. #IMPORTANCE OF SERIAL DILUTION IN RESEARCH ARTICLES SERIES#Thus, serial dilution simply means a series of repeated dilution performed on the same chemical basically to change its concentration. Serial dilution generally refers to selection preformed in the standard growth regimes typically used in the lab: flasks, test tubes, solid media, or 96-well plates. It is usually done if the chemical concentration is too high than the desired composition. Notice the color gradually getting paler as dilution advances.ĭilution is the act of mixing a chemical with other substance, usually distilled water to make it lighter in composition. Principle: A definite weight of solid sample is homogenised aseptically in nine volumes of sterile saline to get a homogenous suspension of bacteria. Serial dilution performed on blue dye solution. Therefore, serial dilution-agar plating method, which enumerates only the viable bacteria cells, is the universally used method for counting living viable cells in different samples.
0 Comments
Leave a Reply. |
AuthorDave ArchivesCategories |